Host species and geographic origin shape the gut bacterial community of Anastrepha fraterculus larvae

SALGUEIRO, J; NUSSENBAUM, A.L.; MILLA, F.; GOANE, L.; RUIZ, M. J.; BACHMANN, G.; VERA, M.T.; TSIAMIS, G.; LANZAVECCHIA, S.B.; SEGURA, D.F.

Congreso; Congreso Americano de Moscas de la Fruta; 2020

The intimate association between host fruit and fruit flies during development envisionsthat fruit should affect the gut bacterial community hosted by larvae. The present work addressesthe effect of host species on gut bacterial diversity in Anastrepha fraterculus larvae consideringthe potential effect of the geographic origin. We considered 2 host fruit species, peach (Prunuspersica) and guava (Psidium guajava), in 2 locations, Concordia (Northeast of Argentina) andHorco Molle (Northwest of Argentina). In each location, 6 trees were randomly selected and 5infested fruits were collected per tree, conforming 120 samples (2 regions x 2 hosts x 6 trees x 5fruits). From each fruit, 5 A. fraterculus larvae were extracted, superficially sterilized and dissectedunder sterile conditions. Middle and posterior gut of 5 larvae were pooled and DNA extraction wasperformed. After amplifying and sequencing V3, V4 hypervariable regions of 16S rRNA gene byIllumina MySeq, the results of our data analysis revealed that gut bacterial community of larvae ismodeled both by host fruit and geographical region. Firstly, larvae collected from guava (G) andpeach (P) showed different bacterial profiles evidenced by β-Diversity, α-Diversity (Shannon),Richness (Chao), and OTUs composition. G presented higher values of Shannon and Chao than P.Secondly, gut bacterial communities from Concordia (C) and Horco Molle (HM) did not differedsignificantly in Shannon or Chao values, but relative abundance pattern analyzed through β-Diversity showed that the bacterial profile differed significantly between C and P. Furthermore,bacterial community of G and P were studied separately and both β-Diversity results showed areaffected significantly by geographic origin. In G, Tatumella was surprisingly abundant, followedby Enterobacter, Wolbachia and Gluconobacter. Lactobacillus was exclusively present in larvaefrom G collected in C. In P Wolbachia was dominant followed by Acetobacter in C, andEnterobacter in HM. Our work confirmed the impact of the host fruit on gut bacterial compositionand reveals the strong effect of geographic origin, found even in the same type of host fruit.