Analysis of the Gut Bacterial Community of Wild Larvae of Anastrepha fraterculus sp. 1: Effect of Host Fruit, Environment, and Prominent Stable Associations of the Genera Wolbachia, Tatumella, and Enterobacter

SALGUEIRO, J; NUSSENBAUM, A.L.; MILLA, F.; ASIMAKIS, E.; GOANE, L.

Frontiers in Microbiology

Frontiers Editorial

Año: 2022

The genus Anastrepha (Diptera Tephritidae) includes some of the most important fruitfly pests in the Americas. Here, we studied the gut bacterial community of 3rd instarlarvae of Anastrepha fraterculus sp. 1 through Next Generation Sequencing (lllumina) ofthe V3-V4 hypervariable region within the 16S rRNA gene. Gut bacterial communitieswere compared between host species (guava and peach), and geographical origins(Concordia and Horco Molle in Argentina) representing distinct ecological scenarios. Inaddition, we explored the effect of spatial scale by comparing the samples collectedfrom different trees within each geographic origin and host species. We also addressedthe effect of fruit size on bacterial diversity. The gut bacterial community was affectedboth by host species and geographic origin. At smaller spatial scales, the gutbacterial profile differed among trees of the same species and location at least inone host-location combination. There was no effect of fruit size on the larval gutbacteriome. Operational Taxonomic Units (OTUs) assigned to Wolbachia, Tatumella andEnterobacter were identified in all samples examined, which suggest potential, nontransientsymbioses. Better knowledge on the larval gut bacteriome contributes valuableinformation to develop sustainable control strategies against A. fraterculus targeting keysymbionts as the Achilles? heel to control this important fruit fly pest.