INVESTIGADORES
RUMIE VITTAR Natalia Belen
artículos
Título:
Caspase-independent apoptosis, in human MCF-7c3 breast cancer cells, following
Autor/es:
NATALIA B. RUMIE VITTAR; JOSEfiNA AWRUCH; KASHIF AZIZUDDIN; VIVIANA RIVAROLA
Revista:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELLULAR BIOLOGY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2010 vol. 42 p. 1123 - 1131
ISSN:
1357-2725
Resumen:
A new water-soluble phthalocyanine derivative, 2,3,9,10,16,17,23,24-octakis(3-aminopropyloxy) phthalocyaninato
zinc II (PoII) was studied as a photosensitizer for photodynamic therapy (PDT) in MCF-7c3 cells.
We report here that PoII and red light induces apoptosis. However, the precise mechanism appears to
differ from that induced by PDT with other known phthalocyanines. The present study provides evidence
that in the case of PoII, caspases do not participate in the apoptotic response. PoII-PDT-treated
cells exhibited chromatin condensation and phosphatidylserine (PS) externalization. In the absence of
light activation, PoII had no detectable cytotoxic effect. An early event upon PoII-PDT was photodamage
to lysosomes, suggesting that they are the primary sites of action. Moreover, the treatment induces Bid
activation, mitochondrial swelling and translocation of apoptosis-inducing factor (AIF) to the nucleus.
An atypical proteolysis of poly(ADP-ribose) polymerase (PARP) indicative of calpain-like activation was
observed. These data support the notion that an alternative mechanism of caspase-independent apoptosis
was found in PoII-photosensitized cells.
We report here that PoII and red light induces apoptosis. However, the precise mechanism appears to
differ from that induced by PDT with other known phthalocyanines. The present study provides evidence
that in the case of PoII, caspases do not participate in the apoptotic response. PoII-PDT-treated
cells exhibited chromatin condensation and phosphatidylserine (PS) externalization. In the absence of
light activation, PoII had no detectable cytotoxic effect. An early event upon PoII-PDT was photodamage
to lysosomes, suggesting that they are the primary sites of action. Moreover, the treatment induces Bid
activation, mitochondrial swelling and translocation of apoptosis-inducing factor (AIF) to the nucleus.
An atypical proteolysis of poly(ADP-ribose) polymerase (PARP) indicative of calpain-like activation was
observed. These data support the notion that an alternative mechanism of caspase-independent apoptosis
was found in PoII-photosensitized cells.
zinc II (PoII) was studied as a photosensitizer for photodynamic therapy (PDT) in MCF-7c3 cells.
We report here that PoII and red light induces apoptosis. However, the precise mechanism appears to
differ from that induced by PDT with other known phthalocyanines. The present study provides evidence
that in the case of PoII, caspases do not participate in the apoptotic response. PoII-PDT-treated
cells exhibited chromatin condensation and phosphatidylserine (PS) externalization. In the absence of
light activation, PoII had no detectable cytotoxic effect. An early event upon PoII-PDT was photodamage
to lysosomes, suggesting that they are the primary sites of action. Moreover, the treatment induces Bid
activation, mitochondrial swelling and translocation of apoptosis-inducing factor (AIF) to the nucleus.
An atypical proteolysis of poly(ADP-ribose) polymerase (PARP) indicative of calpain-like activation was
observed. These data support the notion that an alternative mechanism of caspase-independent apoptosis
was found in PoII-photosensitized cells.
We report here that PoII and red light induces apoptosis. However, the precise mechanism appears to
differ from that induced by PDT with other known phthalocyanines. The present study provides evidence
that in the case of PoII, caspases do not participate in the apoptotic response. PoII-PDT-treated
cells exhibited chromatin condensation and phosphatidylserine (PS) externalization. In the absence of
light activation, PoII had no detectable cytotoxic effect. An early event upon PoII-PDT was photodamage
to lysosomes, suggesting that they are the primary sites of action. Moreover, the treatment induces Bid
activation, mitochondrial swelling and translocation of apoptosis-inducing factor (AIF) to the nucleus.
An atypical proteolysis of poly(ADP-ribose) polymerase (PARP) indicative of calpain-like activation was
observed. These data support the notion that an alternative mechanism of caspase-independent apoptosis
was found in PoII-photosensitized cells.
2,3,9,10,16,17,23,24-octakis(3-aminopropyloxy) phthalocyaninato
zinc II (PoII) was studied as a photosensitizer for photodynamic therapy (PDT) in MCF-7c3 cells.
We report here that PoII and red light induces apoptosis. However, the precise mechanism appears to
differ from that induced by PDT with other known phthalocyanines. The present study provides evidence
that in the case of PoII, caspases do not participate in the apoptotic response. PoII-PDT-treated
cells exhibited chromatin condensation and phosphatidylserine (PS) externalization. In the absence of
light activation, PoII had no detectable cytotoxic effect. An early event upon PoII-PDT was photodamage
to lysosomes, suggesting that they are the primary sites of action. Moreover, the treatment induces Bid
activation, mitochondrial swelling and translocation of apoptosis-inducing factor (AIF) to the nucleus.
An atypical proteolysis of poly(ADP-ribose) polymerase (PARP) indicative of calpain-like activation was
observed. These data support the notion that an alternative mechanism of caspase-independent apoptosis
was found in PoII-photosensitized cells.
We report here that PoII and red light induces apoptosis. However, the precise mechanism appears to
differ from that induced by PDT with other known phthalocyanines. The present study provides evidence
that in the case of PoII, caspases do not participate in the apoptotic response. PoII-PDT-treated
cells exhibited chromatin condensation and phosphatidylserine (PS) externalization. In the absence of
light activation, PoII had no detectable cytotoxic effect. An early event upon PoII-PDT was photodamage
to lysosomes, suggesting that they are the primary sites of action. Moreover, the treatment induces Bid
activation, mitochondrial swelling and translocation of apoptosis-inducing factor (AIF) to the nucleus.
An atypical proteolysis of poly(ADP-ribose) polymerase (PARP) indicative of calpain-like activation was
observed. These data support the notion that an alternative mechanism of caspase-independent apoptosis
was found in PoII-photosensitized cells.
(PoII) was studied as a photosensitizer for photodynamic therapy (PDT) in MCF-7c3 cells.
We report here that PoII and red light induces apoptosis. However, the precise mechanism appears to
differ from that induced by PDT with other known phthalocyanines. The present study provides evidence
that in the case of PoII, caspases do not participate in the apoptotic response. PoII-PDT-treated
cells exhibited chromatin condensation and phosphatidylserine (PS) externalization. In the absence of
light activation, PoII had no detectable cytotoxic effect. An early event upon PoII-PDT was photodamage
to lysosomes, suggesting that they are the primary sites of action. Moreover, the treatment induces Bid
activation, mitochondrial swelling and translocation of apoptosis-inducing factor (AIF) to the nucleus.
An atypical proteolysis of poly(ADP-ribose) polymerase (PARP) indicative of calpain-like activation was
observed. These data support the notion that an alternative mechanism of caspase-independent apoptosis
was found in PoII-photosensitized cells.