Estrogen Receptor beta regulation by estradiol and its impact on pituitary cell proliferation

PEREZ P.; PICECH F.; SOSA L.; PETITI J.P.; DE PAUL A.L.; TORRES A. I.; GUTIÉRREZ S.

Mar del Plata

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

Estrogens are involved in the modulation of multiple cellular processes, exerting their effects through specific estrogen receptors (ER) α and β. ER expression levels strongly influence the estrogenic activity in a tissue or cell. The aim of this study was to determine the pituitary ERβ expression, analyze its role and the molecular mechanisms involved in the pituitary cell proliferation. For this purpose the following models were used: 1)in-vivo: anterior pituitary glands from Wistar rats (females at different estrous cycle stages, ovariectomized (OVX) and chronically stimulated with estradiol (E2) for 20, 40 and 60 days and male rats) and 2)in vitro: GH3 cells transfected to ERβ overexpression. E2 and ERβ (PPT) and ERβ (DPN) agonists were employed. Proliferation (BrdU technique) and DNA content (Propidium Iodide) were quantified. ERβ and β, cyclin D1 (CD1), Akt (t and p) and PTEN expression were determined by Western blot and quantified by flow cytometry. Subcellular localization was analyzed by immunofluorescence and electron microscopy. ANOVA-Tukey was used (p