Prostatic stromal cell response to LPS exposition

LEIMGRUBER C.; QUINTAR A.; PETITI J.P.; MALDONADO C. A.

Buenos Aires

Jornada; X Jornadas Anuales de la Sociedad Argentina de Biología; 2008

We previously demonstrated that bacterial prostatitis produces changes in smooth muscle cells (SMC) phenotype. Considering the pivotal role of these cells in gland homeostasis maintenance through stromal/epithelial interactions, our objective was to analyze their response to lipopolysacharide (LPS). Primary cultures of stromal prostatic cells from Wistar rats were performed with cells being plated in selective media MCDB 131. First, the cells were pre-incubated with TGFb1 (1ng/mL) from 7 to 9 days in order to evaluate the homogeneity of cell population. TGFb1 treatment resulted in the increment of smooth muscle a-actin cells compared with control cells without treatment. Afterwards, TGFb1-treated stromal cells, were incubated with LPS (1ng/mL) for 24 hs. Cells were processed for morphological analysis and ICQ at optical and electron microscopy level. We evaluated expression of cytoesqueleton proteins, innate immune molecules (TLR4, TNFa) and NFkB translocation. LPS induced changes in actin distribution which appeared clumped in patches. Secretory-like granules of low electron density appeared dispersed in the cytoplasm. Moreover, TLR4 and TNFa expression increased in cytoplasm and NFkB ?ntranslocated to the nucleus. These observations let us conclude that SMC respond to LPS, evidencing cellular activation to express innate immunity molecules and extracellular matrix molecules. Results stand out the importance of SML that demonstrated to take active part in prostate response to inflammatory stimuli.