Genome mining of lipolytic exoenzymes from Bacillus safensis S9 and Pseudomonas alcaliphila ED1 isolated from a dairy wastewater lagoon

FLORENCIA A. FICARRA; IGNACIO SANTECCHIA; SEBASTIÁN H. LAGORIO; SERGIO ALARCÓN; CHRISTIAN MAGNI; MARTÍN ESPARIZ

ARCHIVES OF MICROBIOLOGY

SPRINGER

Lugar: Berlin; Año: 2016

0302-8933

Dairy production plants produce highly polluted wastewatersrich in organic molecules such as lactose, proteins and fats. The latter generallyresult in a low overall performance of the treatment system. In this study, awastewater dairy lagoon was used as microbial source and different screeningstrategies were conducted to select 58 lipolytic microorganisms. Exoenzymes and RAPD analyses revealed genetic and phenotypicdiversity among isolates. Bacillus safensis,Pseudomonas alcaliphila, and the potential pathogens, B. cereus, Aeromonas and Acinetobacter were identified by 16S-rRNA, gyrA, oprI and/or oprL sequence analyses. Five out of 10 selected isolates produced lipolyticenzymes and grew in dairy wastewater. Based on these capabilities and their safety,B. safensis S9 and P. alcaliphila ED1 were selected and their genome sequences determined. The genome of strain S9 and ED1 consisted of 3,794,315 and5,239,535 bp and encoded for 3,990 and 4,844 genes, respectively. Putative extracellularenzymes with lipolytic -12 and 16- proteolytic -20- or hydrolytic -10 and 15-activity were identified for S9 and ED1strains, respectively. These bacteria also encoded othertechnological relevant proteins such as amylases, proteases, glucanases,xylanases and pectate lyases.