Efficacy and toxicology of silver nanoparticles as a component wound of healing dressing in zebrafish larvae and embryo

SOSA AYELEN M; IGARTÚA DANIELA; GARCÍA LORENA; OCAMPO ALEJANDRO; ALONSO SILVIA DEL V; PRIETO JIMENA; MARTINEZ CAROLINA

Congreso; Reunión de Biociencias 2020 - SAIC, SAI, SAFI; 2020

Silver nanoparticles (AgNPs) have an important role in nanotechnol-ogy since they possess exceptional bactericidal activity. Increasingapplications are the AgNP-based wound dressing, providing a largesurface area with high bactericidal efficacy. We aimed to design afilm of hydrogel, composed of autochthonous and low cost poly-mers. Within the film, oil-in-water emulsions will be used to encap-sulate and delivery AgNPs, vitamins, anesthetic, and anti-inflamma-tories. This novel device for the treatment of wounds and burns willbe enriched with supplements focused in the reduction of pain andin the acceleration of healing process. As a first step, we will carryout an analysis of efficacy of the AgNPs in opportunistic bacteriascommonly found on the skin, and toxicology of the AgNPs in zebraf-ish embryo and larvae. Results will help to select a concentrationto next use in the emulsion. To evaluate effectiveness of AgNPs,Staphylococcus aureus and Pseudomonas aeruginosa were incu-bated with 1.6-60 ppm AgNPs, and minimal inhibitory concentration(MIC) was determined. To evaluate toxicology of AgNPs, Zebrafishembryo and larvae were incubated with 0.1-100 ppm AgNPs, thenstudies of LC50, acute toxicity (mortality), teratogenicity (hatchingrate and morphology), general developmental abnormalities andspecific tissue toxicity (brain and heart function) were conducted.The bacterial inhibition indicated a high susceptibility of Staphylo-coccus aureus and Pseudomonas aeruginosa from 30 ppm. It wasobserved that AgNPs, in the highest concentrations, caused a delayin the development of embryos, morphological abnormalities in thelarvae, decreased swimming and heart rate. In the larval lethalitystudy, it was observed that the LC50 at 1 hs post-incubation was 683ppm, and at 48 hs post-incubation, it was 108 ppm. The comparisonof in vitro and in vivo results allows us to find a functional and harm-less AgNPs dose.