Effect of transition metals in synaptic damage induced by amyloid beta peptide

URANGA, ROMINA MARÍA; GIUSTO, NORMA MARÍA; SALVADOR, GABRIELA ALEJANDRA

NEUROSCIENCE

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2010 vol. 170 p. 381 - 389

0306-4522

<!-- /* Font Definitions */ @font-face {font-family:"MS Mincho"; panose-1:2 2 6 9 4 2 5 8 3 4; mso-font-alt:"ＭＳ 明朝"; mso-font-charset:128; mso-generic-font-family:modern; mso-font-pitch:fixed; mso-font-signature:-536870145 1791491579 18 0 131231 0;} @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 415 0;} @font-face {font-family:"@MS Mincho"; panose-1:2 2 6 9 4 2 5 8 3 4; mso-font-charset:128; mso-generic-font-family:modern; mso-font-pitch:fixed; mso-font-signature:-536870145 1791491579 18 0 131231 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"MS Mincho"; mso-ansi-language:ES-AR; mso-fareast-language:ES;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ascii-font-family:Calibri; mso-fareast-font-family:Calibri; mso-hansi-font-family:Calibri;} @page WordSection1 {size:612.0pt 792.0pt; margin:72.0pt 72.0pt 72.0pt 72.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.WordSection1 {page:WordSection1;} --> The amyloid β‑peptide (Aβ), which is thought to be the major cause of Alzheimer?s disease, is known to be capable of aggregating in different states: soluble monomers and oligomers, and insoluble aggregates. The Aβ aggregation state as well as its toxicity has been related to the interaction between the peptide and transition metals such as iron and copper. However, this relationship, as well as the effects of Aβ on the synaptic endings, is not fully understood. The aggregation states of Aβ in the presence of iron and copper, as well as their effects on synaptic viability and signaling were investigated in this work. During acute incubation treatments (5 min ? 4 h), Aβ/metal impaired mitochondrial function to the same extent as has been observed with the metal alone. However, in the presence of Aβ/iron (10 and 50 μM), plasma membrane integrity was disrupted to a greater extent than when generated by either iron or Aβ alone, indicating that the membrane constitutes the first target of synaptic injury. Akt activation by Aβ/iron was evident after 5 min of incubation and was higher than that observed in the presence of the metal alone. This activation was barely detected after 4 h of incubation, demonstrating that there is no correlation between the extent of synaptic damage and the activation of this kinase. ERK1/2 activation profile was different from that observed for Akt. Accordingly, the presence of Aβ/metal could differentially modulate the activity of these kinases. This work shows evidence of the initial events locally triggered at the synapse by Aβ and transition metals. As synapses have been proposed as the starting point of Aβ/metal‑triggered events, the characterization of early mechanisms occurring in models that mimic Alzheimer?s disease could be important for the search of unexplored therapeutics tools.