Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV).

KOBAYASHI KEN, RABINOWICZ PABLO, BRAVO-ALMONACID FERNANDO, HELGUERA MARCELO, CONCI VILMA, LOT HERVE, MENTABERRY ALEJANDRO.

ARCHIVES OF VIROLOGY

Springer-Verlag

Año: 1996 vol. 141 p. 2277 - 2287

0304-8608

Summary. Partial genomic sequences from an unknown garlic potyvirus andfrom an onion isolate of the onion yellow dwarf potyvirus (OYDV) wereobtained. Comparison of the deduced amino acid sequences showed a similarityof 88% between the respective viral coat proteins. The garlic potyvirus coatprotein was expressed in E. coli cells, purified, and subjected to Western blotanalysis using antibodies raised against different garlic-infecting viruses. Theexpression protein was consistently recognised by anti-OYDV antibodies anddid not react with antibodies specific for leek yellow stripe potyvirus (LYSV),garlic common latent carlavirus (GCLV) and shallot latent carlavirus (SLV).Besides, the garlic potyvirus coat protein was obtained as a fusion protein andused as antigen to produce polyclonal antibodies. These antibodies reacted withpurified OYDV virions, but failed to recognise LYSV particles. In the light of thisevidence the garlic potyvirus was identified as the garlic strain of OYDV.