Bioactive sphingolipid ceramide 1 phosphate (C1P) prevents ovarian aging by improving microvasculature

SCOTTI LEOPOLDINA; PASCUALI NATALIA; OUBIÑA, GONZALO; MENA AGUSTINA; ABRAMOVICH DALHIA; NEGROTTO SOLEDAD; PARBORELL FERNANDA

Congreso; International Vascular Biology Meeting; 2018

1)Endothelial progenitor cells (EPCs) were detected in peripherial blood 7 days post C1P administration in ovaries from aged mice.2)C1P increased the periendothelial cells area and VEGF-A levels in ovaries from aged mice.3)C1P in FF from aged patients increased the endothelial cells migration but had no effect on VEGF-A levels. As women increasingly postpone childbearing, many aged females become infertile. Activation of ovarian angiogenesis has emerged as a strategy to prevent ovarian aging. Objectives: 1) Evaluate the local effect of sphingolipid C1P on EPCs mobilization and on ovarian angiogenesis in aged female mice. 2) Analyze the angiogenic potential of follicular fluid (FF) from patients in advanced reproductive age (ARA) in the presence of C1P in endothelial cells (ECs). Aged mice (26-31 weeks) received intraovarian C1P (10µl/ovary; 50µM). Animals were sacrificed 2 and 7 days later. Prior to surgery and sacrifice, peripheral blood (PB) was extracted to evaluate EPCs mobilization by flow cytometry (VE-Cadherin+, Sca-1+). Immunohistochemistry (α-SMA) and Western blot (VEGF-A) were performed on ovaries. ECs were incubated with FF from ARA (38-42 years) and control (