Phototherapy in female reproductive medicine: effects on ovarian function and possible uses in oncofertility.

OUBIÑA, GONZALO; SCOTTI LEOPOLDINA; LA SPINA FLORENZA; BUFFONE MARIANO; HIGUERA JAVIER; ABRAMOVICH DALHIA; PARBORELL FERNANDA

Concon

Congreso; XXIX Reunión Anual de la Sociedad Chilena de Reproducción y Desarrollo; 2018

It is known that LLLT has beneficial effects on several processes including wound healing, pain and inflammation. LLLT modulates biological functions, including cell proliferation, apoptosis and angiogenesis, which in the ovary are intimately related to fertility. Premature Ovarian Failure (POF) is characterized by the disappearance of ovarian follicles in young women, which may be caused by chemotherapy. Current treatments for POF, mainly hormone therapies, are not completely effective. The present study proposes photobiomodulation as a strategy to protect the ovary in cancer patients undergoing chemotherapy. The objectives were: to evaluate the in vivo effect of LLLT on ovarian function a) in adult mice, and b) in POF model induced by chemotherapy. For objective a, LLLT (100 and 200 J/cm2) was applied to F1 mice (BalbC x C57 / BL6) (8 weeks). For objective b), POF model was induced with cyclophosphamide (CTX, 75mg/weight) in F1 mice and LLLT (200 J/cm2) was applied. a) Adult mice: LLLT (200 J/cm2) increased the % of primary and preantral follicles, whilst decreasing the percentage of corpora lutea compared to control ovaries (p