INVESTIGADORES
ABRAMOVICH Dalhia Nurit
artículos
Título:
Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment.
Autor/es:
ABRAMOVICH DALHIA; RODRIGUEZ CELIN ALEJANDRA; HERNANDEZ FATIMA; TESONE MARTA; PARBORELL FERNANDA
Revista:
REPRODUCTION
Editorial:
Bioscientifica
Referencias:
Año: 2009 vol. 137 p. 309 - 320
ISSN:
1470-1626
Resumen:
This study investigated the protein expression and cellular
localization of ANGPT1, ANGPT2, and their receptor TEK, as well as
vascular endothelial growth factor A (VEGFA) and its receptor KDR
(VEGFR2) during folliculogenesis. To obtain follicles at different
stages for immunochemistry and western analyses, we used prepubertal
untreated, diethylstilbestrol- and equine chorionic
gonadotropin-treated rats. To confirm that these hormonal treatments
reflect physiological change, we used non-treated adult rats. No
expression of ANGPT1 was observed in granulosa cells (Gc) from immature
hormone-treated and non-treated rats at any follicular stage. By
contrast, ANGPT1 expression in theca cells (Tc) increased with
follicular maturation. ANGPT2 protein was either absent or weakly
expressed in Gc at all follicular stages. In Tc, minimal expression of
ANGPT2 protein was detected in the preantral follicle (PF), whereas it
was stronger in the early antral follicle (EAF) and preovulatory
follicle (POF). TEK staining was absent in Gc but was intense in Tc at
every follicular stage. Staining for VEGFA was either absent or weakly
present in Gc and Tc in PF and EAF, although in POF it was stronger in
Gc and Tc. Staining for KDR was absent in Gc and very low in Tc from
PF. Gc and Tc of EAF showed positive staining for KDR and in POF the
staining was stronger. These results were confirmed by western
immunoblot. A similar pattern of expression of these proteins was
observed in cycling rats. In conclusion, we observed that the protein
expression of ANGPT1, ANGPT2, VEGFA and their receptors increased
during follicular development in rats.