INVESTIGADORES
PEREZ DE ROSAS Alicia Raquel
artículos
Título:
Macrogeographic and microgeographic genetic structure of the Chagas disease vector Triatoma infestans (Hemiptera: Reduviidae) from Catamarca, Argentina.
Autor/es:
ALICIA R. PÉREZ DE ROSAS; ELSA L. SEGURA; LAURA FICHERA; BEATRIZ A. GARCÍA
Revista:
GENETICA
Editorial:
Springer
Referencias:
Año: 2008 vol. 133 p. 247 - 260
ISSN:
0016-6707
Resumen:
The genetic structure in populations of the
Chagas disease vector Triatoma infestans from six localities
belonging to areas under the same insecticide treatment
conditions of Catamarca province (Argentina) was examined
at macrogeographical and microgeographical scales. A total
of 238 insects were typed for 10 polymorphic microsatellite
loci. The average observed and expected heterozygosities
ranged from 0.319 to 0.549 and from 0.389 to 0.689,
respectively. The present results confirm that populations of
T. infestans are highly structured. Spatial genetic structure
was detectable at macrogeographical and microgeographical
levels. Comparisons of the levels of genetic variability
between two temporal samples were carried out to assess the
impact of the insecticide treatment. The genetic diversity of
the population was not significantly affected after insecticide
use since different genetic parameters (allele number,
observed and expected heterozygosities) remained stable.
However, loss of low frequency alleles and not previously
found alleles were detected. The effective population size (Ne) estimated was substantially lower in the second temporal sample than in the first; nevertheless, it is possible that the size of the remnant population after insecticide treatment was still large enough to retain the genetic diversity. Very few individuals did not belong to the local T. infestans populations as determined by assignment analyses, suggesting a low level of immigration in the population. The results of the assignment
and first-generation migrant tests suggest male-biased dispersal at microgeographical level.