INVESTIGADORES
ALBA SOTO Catalina Dirney
artículos
Título:
Lineage, maturity, and phenotype of uterine murine dendritic cells throughout gestation indicate a protective role in maintaining pregnancy.
Autor/es:
BLOIS, SANDRA; ALBA SOTO, CATALINA D; TOMETTEN, MARIEKE; KLAPP, BURGHARD; MARGNI, RICARDO; ARCK, PETRA
Revista:
BIOLOGY OF REPRODUCTION
Editorial:
SOC STUDY REPRODUCTION
Referencias:
Lugar: Winsconsin (USA); Año: 2004 vol. 70 p. 1018 - 1023
ISSN:
0006-3363
Resumen:
PROBLEM: DBA/2J-mated CBA/J female mice are prone to a high
incidence of fetal abortions. This fetal wastage can be dramatically
reduced by immunizing the female mice with BALB/c, but not with
DBA/2J spleen cells during early gestation. Nevertheless, the
underlying mechanisms remain to be elucidated. Recently, dendritic
cells (DC) have been described at the feto-maternal interface in the
human uterus. In this work, we studied the effect of adoptive transfer
of DC on the maintenance of pregnancy in the CBA/J · DBA/2J
model.
METHODS: Bone marrow-derived DC were generated from virgin
female CBA/J mice (68 weeks old). CBA/J females were inoculated
with DC twice before mating. Four different experimental groups were
included: (i) no treatment control, (ii) mice injected with culture
medium [granulocytemacrophage colony-stimulating factor (GMCSF)],
(iii) immunized with DC and (iv) immunized with paternal
DBA/2J antigens lisate-pulsed DC, n ¼ 5.
RESULTS: The control abortion rate was 23.8%, and with GM-CSF
alone was 17.6%. Following inoculation of syngeneic DC abortion
rates were reduced to 2.2%, but protection was short-lived. Abortion
rates with DC pulsed with DBA/2J antigens was 5%. Serum of
interleukin (IL)-6 levels were lower in the latter two groups up to the
time of abortion. The kinetics of immunoglobulin G asymmetric
antibodies synthesis was modified, but there was no correlation
between asymmetric antibodies production and the lowering of
abortions rates.
CONCLUSION: Syngeneic DC prevented abortions and this was
linked to a decrease in IL-6 levels, but not with levels of asymmetric
antibodies.