INVESTIGADORES
FELDMAN Mariana Laura
congresos y reuniones científicas
Título:
. Expression analysis of genes potentially involved in induced resistance mediated by phosphite in potato.
Autor/es:
FELDMAN M.L.; MACHINANDIARENA MF; DALEO GR; ANDREU AB
Lugar:
Potrero de Funes-San Luis
Reunión:
Congreso; Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011
Institución organizadora:
SAIB
Resumen:
Preliminary results of microarray analysis from potato sprouts treated or not with phophites (KPhi), showed that 26 genes were upregulated in the treated ones. These genes were classified into 5 groups: plant defense, metabolism, abiotic stress, transcription factors and unknown genes. Within those we have selected 3 genes (Cullin1, ERD15 & BCYA) known to be involved in induced resistance (IR) and we validated them by qRT PCR. The aim of this work was to analyse their expression in leaves treated or not with KPhi, infected or not with Phytophtora infestans (Pi). Semi quantitative RT PCR showed that the 3 genes were expressed only in KPhi treated leaves. To analyze if Pi infection acts sinergically with KPhi, we quantify their expression 24 and 48 hs after infection, in KPhi treated or non treated leaves. After 24 hs, BCYA and Cullin 1 increased their expression in infected leaves, with respect to the non infected ones, however KPhi treatment did not act sinergically, moreover it was reduced for Cullin 1. ERD15 expression did not change with respect to the non infected leaves, but it increased when KPhi was applied. Their expression decreased after 48 hs treatments. These results could help to understand the battery of responses involved in IR mediated by KPhi