PARTICIPATION OF GUT MICROBIOTA ON THE METABOLIC CHANGES INDUCED BY HIGH FAT DIET

MARCONE, PAULA; RUBINSTEIN ROXANA; GEREZ ESTHER; PROCHNIK, ANDRES; BURGUEÑO AL; GENARO, ANA M.; WALD, MIRIAM RUTH

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC)

Environmental factors, such as a fatenriched diet are among the causes of the great prevalence ofobesity and type 2 diabetes in the population. Recent studieshave shown that diet-induced alterations the gut microbiotacomposition play a pivotal role in the development of obesity.Changes in the predominant gut bacterial phylums:Bacteroidetes and Firmicutes (B-F) characterizes differentmetabolic phenotypes. Our objective is to study in a high fat diet(HFD) feeding model of obesity if the treatment with probioticsinduces changes in glycidic metabolism and B-F DNA. Four weekold male C57B6/6J mice were fed with a normal chow diet (fatcontent: 7.5 g/100 g) or an HFD diet (fat content: 31 g/100 g,butter and lard). When HFD mice reached a 5 % weight gain withrespect to the controls (p= 0.07, n= 12) and a cumulativeincrease in food intake of 19 % kcal (week 16), probiotictreatment was started. We used two type of probiotics (P1 andP2) in two concentrations: 107 CFU and 108 CFU, supplied indrinking water. Genomic DNA of gut microbiota was isolated fromfeces samples and were analyzed by real time PCR reactionsusing selective primers. HFD induced an increment in basal (Gb)and after 2 hours of glucose administration (G2h) glycemia.Lower dose probiotic treatment did not produce changes in thoseparameters, however the higher dose induced an improvement inGb and G2h (ANOVA Gb p= 0.0204, G2h p= 0.022, n= 4) beingP2 most effective. No changes were observed in body weight andfood intake. Concerning gut microbiota, we observed a nonsignificant increase in the Bacteroidetes DNA under treatmentwith HFD and P1 (interaction diet probiotic p= 0.06, n= 4). Weconclude that probiotic treatment improved metabolicparameters that were altered during HFD treatment. These datasuggest the importance of gut microbiota as a therapeutic targetin the treatment of obesity complications