Anisodamine production by Argentinean and Colombian Brugmansia candida in vitro cultures

CARDILLO, ALEJANDRA BEATRIZ; OTALVARO, ANGELA MARíA; CALABRO LOPEZ, ARIEL; VELáSQUEZ, MARIO ENRIQUE; RODRIGUEZ TALOU, JULIAN; GIULIETTI, ANA MARIA

Rosario, Argentina

Congreso; VII Simposio Nacional de Biotecnologia REDBIO-Argentina; II Congreso Internacional-REDBIO-Argentina; 2009

REDBIO FAO

Anisodamine (6b-hydroxyhyoscyamine), scopolamine, and hyoscyamine belong to the tropane alkaloids group characterized by its anticholinergic activity. Hyoscyamine and scopolamine were traditionally used as pharmaceuticals. Nowadays, anisodamine is gaining attention due to the wide range of therapeutic applications described for this tropane alkaloid which includes the control of toxic shock, septic shock, and orghanophosphorus poisoning, treatment of microvascular diseases, glomerulonephritis, rheumatoid arthritis, gastrointestinal colic, hemorrhagic necrotic enteritis and eclampsia. In addition to the medical applications described, the use of anisodamine as an anticholinergic agent present certain advantages such as the fact to be less toxic than atropine and the fewer negative effects over Central Nervous System than scopolamine. In this work, the production of anisodamine by in vitro cultures of Brugmansia candida (Argentinean and Colombian samples) was study. This alkaloid was estimated in different organs of in vitro germinated seedlings as well as in hairy roots obtained from both origins. Colombian roots presented the highest content of tropane alkaloids, being anisodamine the main alkaloid measured. In leaves, no significant differences were observed for Argentinean and Colombian samples being scopolamine the main alkaloid detected. Anisodamine, scopolamine and hyoscyamine content in Argentinean hairy roots was significantly higher than in Colombian ones, being anisodamine the main alkaloid detected. According to the results obtained and the increasing interest in the use of in vitro cultures for large scale secondary metabolite production, Argentinean B. candida hairy root cultures appear to be a promising system for the anisodamine production.