CARDILLO Alejandra Beatriz
capítulos de libros
Tropane alkaloid production by the establishment of hairy root cultures of Brugmansia candida and elicitation
CARDILLO, ALEJANDRA BEATRIZ; PERASSOLO, MARÍA; MINOIA, JUAN MAURICIO; RODRIGUEZ TALOU, JULIAN; GIULIETTI, ANA MARIA
Hairy Root Cultures (HRCs) based Applications
Año: 2020; p. 123 - 132
The medicinal use of tropane alkaloids is well established and has a long history of application, according to the anticholinergic activity of these compounds. Among them, hyoscyamine, anisodamine (6b-hydroxyhyoscyamine) and scopolamine are the most important ones from a therapeutical point of view. Although the chemical synthesis of these alkaloids is possible, its complexity, costs and low quality of the compounds obtained make it a non-viable strategy for a commercial production. For the reason mentioned above, tropane alkaloids are still extracted from plants grown in green houses belonging to species of Solanaceae family including, Hyoscyamuns niger L., Anisodus tanguticus, Scopolia tangutica Maxim, Atropa belladonna, and several Datura species. In the last decade much effort has been invested in the development of cost-effective strategies for their production. Tropane alkaloids production by in vitro culture techniques, such as hairy roots, is an interesting alternative since it guarantees a stable and continuous production throughout the year, independent of the presence of pathogens as well as the environmental conditions. This chapter will focus and discuss the establishment of hairy root cultures for the production of tropane alkaloids in order to replace the isolation from the natural source. In addition, recent progress achieved in the application of elicitation strategies for improving their release to the culture medium will be analyzed.