Feeding increases MyoD and PCNA expression in myogenic progenitor cells of Notothenia coriiceps.

JULIE C. BRODEUR; LLOYD S. PECK; IAN A. JOHNSTON

JOURNAL OF FISH BIOLOGY

Año: 2002 vol. 60 p. 1475 - 1485

0022-1112

Oxygen consumption and ammonia excretion of the Antarctic fish Notothenia coriiceps (18·4 cm LF) increased respectively two and fourfold above fasting levels 24 h after feeding with a single meal of shrimps (5·5 to 7·5% of body mass), and remained elevated for 120 h. In fasted fish, c-met positive cells in the fast muscle represented 5·5% of the total number of myonuclei. The number of c-met positive cells staining for the proliferating cell nuclear antigen was increased by 60% both 24 and 96 h after the meal, while the number of cells expressing the myogenic transcription factor, MyoD, was increased by 20% after 24 h, and by 44% after 96 h. The total numbers of c-met positive cells and cells expressing myogenin were not significantly altered 96 h following feeding. The results are consistent with an activation of myogenic progenitor cells proliferation by feeding but suggest a relatively long cell cycle time.