Measuring and Perturbing Ferroptosis in Plants.

AYELEN M. DISTÉFANO; FERNANDA MARCHETTI; EDUARDO ZABALETA; GABRIELA C. PAGNUSSAT

Plant Proteases and Plant Cell Death. Methods in Molecular Biology, vol 2447. Humana, New York,

Springer

Año: 2022; p. 185 - 192

Ferroptosis is an oxidative iron-dependent cell death that was recently described in vertebrates, invertebrates, fungi, plants, and bacteria. In plants, ferroptosis has been reported in response to heat shock in roots of 6-day-old Arabidopsis thaliana seedlings. Generally, all biochemical and morphological ferroptosis hallmarks are conserved between animals and plants. Here, we describe a protocol to induce and quantify ferroptosis in plants based on the analysis of dead cells with a Sytox Green stain. Furthermore, heat shock induced cell death is prevented by using specific ferroptosis inhibitors.