FOXO3A EXPRESSION VARIES DURING MAMMARY GLAND TISSUE REMODELING AND WOULD BE PARACRINALY MODULATED BY GNRH IN FEMALE VIZCACHAS (LAGOSTOMUS MAXIMUS)

MARÍA CLARA CORSO, SANTIAGO ANDRÉS CORTASA, ALEJANDRO RAÚL SCHMIDT, SOFÍA PROIETTO, PABLO IGNACIO FELIPE INSERRA, ALFREDO DANIEL VITULLO, VERÓNICA BERTA DORFMAN; HALPERIN J

Congreso; Reunion Anual de Biociencias 2021; 2021

SAIC, SAI, NANOMED, AAFE

FoxO3a belongs to the Forkhead box class O (FoxO) transcriptionfactor family which modulates many metabolic processes, rangingfrom apoptosis, cell cycle progression, stress resistance to fine endocrineregulation of reproductive organs. For the latter, it has beenreported that GnRH transactivates genes critical for gonadal functionthrough another member of the FoxO family: FoxO1. Since wehave recently demonstrated local expression and paracrine action ofGnRH in mammary glands (MG) of pregnant vizcachas, we aimedto investigate FoxO3a expression throughout pregnancy and its relationshipwith GnRH by immunohistochemistry. In addition, FoxO3expression levels were assessed in MG explants of term-pregnantfemales stimulated with a GnRH analogue. FoxO3a reactivity wasdetected in all the analyzed stages (early-, mid- and term-pregnant,non-pregnant, and lactating), always in the secretory epithelium ofthe MG. However, both the percentage of FoxO3a immunoreactivearea (%IR) and its immunoreactive optic density were significantlyhigher in early- and non-pregnant than those of the other groups(p