A PROTEOMIC STUDY REVEALS NEW MARKERS OF PROLACTIN-MODULATED OVARIAN FUNCTION IN PLAINS VIZCACHAS

CORTASA, SANTIAGO, FEEHAN, KEVIN, SCHMIDT, ALEJANDRO, VITULLO, ALFREDO, DORFMAN, VERÓNICA; HALPERIN J

Congreso; Reunion Anual de Biociencias 2021; 2021

SAIC, SAI, NANOMED, AAFE

Prolactin (PRL) modulates the expression of the LH receptor inthe ovary and, thus, the cascades of steroidogenic enzymes thatsynthesize and produce ovarian steroids. When pathological hyperprolactinemiaoccurs, the pulsatile release of GnRH decreases andalters the pituitary production of FSH and LH. Furthermore, it directlyimpairs the endocrine activity of ovarian follicles. To analyze whichovarian factors, aside to the aforementioned enzymes, respond toa high PRL environment, an ovarian proteomic study of vizcachaswith sulpiride-induced hyperprolactinemia was performed. For this,ovarian protein extracts from hyperprolactinemic (HPRL) and control(CTL) females (n=5 per group) were used. Briefly, equal amountsof protein were analyzed using MALDI-TOF/MS and then, LC-ESI/MS (Orbitrap). The resulting peptides were identified with ProteomeDiscoverer Software using the Rodentia UniProt Database,and functional enrichment analysis was performed using DAVID,STRING and FunRich softwares. Proteins differentially expressedin each treatment were depicted in a volcano plot (t-test, p