Mammary gland-specific regulation of GnRH and GnRH-receptor gene expression is likely part of a local autoregulatory system in female vizcachas (Chinchillidae: Rodentia).

CORSO MC1,2, PROIETTO S1,2, CORTASA SA1,2, SCHMIDT AR1,2, FEEHAN KT1, FIDEL V1, INSERRA PIF1,2,FERNÁNDEZ M. 3 CWIRENBAUM R1., VITULLO AD; DORFMAN, VERÓNICA BERTA; HALPERIN J

Mar del Plata

Congreso; XXIX Annual Meeting Sociedad Chilena de Reproducción y Desarrollo. septiembre 2018, Concón, Chile; 2019

Sociedad Argentina de Investigación Clínica

Our laboratory has recently reported gonadotropin-releasing hormone (GnRH) protein expression in mammary gland (MG) epithelial cells of vizcachas during pregnancy and lactation. In addition, we also shown that prolactin (PRL) modulates GnRH MG content. The present work aims to study GnRH gene expression in MG and also, to explore a possible GnRH-autocrine mechanism over this tissue. We amplified GnRH, GnRH-receptor (GnRH-R), early growth response factor 1 (Egr-1), PRL, PRL-receptor (PRL-R) and α-lactalbumin by RT-PCR using mRNA from MG at different stages of pregnancy and lactation. We established local transcription of GnRH at all the analyzed stages and the amplicon sequencing confirmed GnRH identity. Maximum transcription of GnRH occurred at early pregnancy and this coincides with maximum transcription of GnRH-R (p