2,4-dichlorophenoxyacetic acid induced oxidative stress in cerebellar granule cells in cultures and amphetamine reduced this effect

BONGIOVANNI BETTINA; BRUSCO ALICIA; FERRI ALEJANDRO; RASSETTO MAURICIO; LOPEZ MARGARITA; EVANGELISTA DE DUFFARD ANA MARIA; DUFFARD RICARDO

Pucon

Congreso; III Neurotoxicity Society Meeting; 2007

Neurotoxicity Society

2,4-Dichlorophenoxyacetic acid (2,4-D), a worldwide-used herbicide, has been associated with a wide range of adverse health effects -from embryotoxicity and teratogenicity to neurotoxicity- on human and different animal species. In the present work, neuronal morphology and biochemical events in rat cerebellar granule cell cultures (CGC) have been analyzed in order to determine which are the mechanisms involved in the cell death induced by 2,4-D. In addition, amphetamine (AMPH), that has showed accelerate recovery of several functions in animals with brain injury, has been utilized as pharmacological tool. This study showed a drastic decrease in the viability of CGC exposed to 2,4-D and apoptotic and necrotic cells were observed. An increased in the reactive oxygen species level, a decreased glutathione content and CAT activity, as well as an increase in the selenio-glutathione peroxidase activity, with respect to the control group, were also detected. In this way, the apoptosis produced by 2,4-D could be unleashed for the oxidative imbalance in CGC. When the cells were exposed to 2,4-D plus d-AMPH a diminution of the oxidative stress, cytoplasmatic alterations and no apoptotic figures were observed.