Amphetamine decrease the oxidative stress produced by 2,4-dichlorophenoxyacetic acid

BONGIOVANNI BETTINA; FERRI ALEJANDRO; KONJUH CINTIA; RASSETTO MAURICIO; EVANGELISTA DE DUFFARD ANA MARIA; DUFFARD RICARDO

Mar del Plata

Congreso; I Congreso Conjunto de Cs Biomédicas. XIX Reunión Anual de la Sociedad Argentina de Neuroquímica; 2004

Sociedad Argentina de Neuroquímica (SAN)

2,4-Dichlorophoxyacetic (2,4-D) acid and derivatives are herbicides widely used in Argentine and other of the world. Exposure to 2,4-D, its ester and salt formulations, have been associated with a range of adverse health effects in humans and different animal species, from embryotoxicity and teratogenicity to neurotoxicity. In previously work of our laboratory, it has been demonstrated that after 24 and 48 hs of treatment with 2,4-D there is an induction of apoptosis in rat cerebellar granule cell cultures, a process generally believed to be mediated by oxidative stress. Furthermore, we demonstrate that the neurotoxicity produced by 2,4-D in cerebellar granule cell cultures decrease with addition to very little Amphetamine. At the present time, one of toxicity mechanism postulated for this herbicide involve the oxidative stress. The aim of the present work was to determinate in rat cerebellar granule cell cultures, exposed to 2,4-D (1mM) and/or Amphetamine (1 and 10 uM) for 24 and 48 hs, reactive oxygen species (ROS) using a fluorescent compound (DCF), catalase (CAT) activity by method described by Beers and Sizer and both Mn- and Cu, Zn-superoxide dismutase (SOD) activity by the method of Fridovich, and production of NO by Griess’ method.We observed that the 2,4-D produced increase the ROS after 24 and 48 hs of treatment, while both treatment with Amphetamine and Amphetamine/2,4-D did not present modification with the control group. On the other hand, CAT activity decrease with 2,4-D and with both drugs after 48 hs to exposure, but the decrease was significantly lower than with 2,4-D alone. However, SOD activities (both Mn- and Cu, Zn) did not affect. Furthermore, production of NO after 24 hs of treatment with 2,4-D was higher than respective control values and amphetamine group. However, the treatment with both drugs presented lower production of NO than the treatment with the herbicide alone. In conclusion, the data in this work support the concept that 2,4-D produced increases the reactive species and the little dose of amphetamine decreases these levels. This fact would be important as mechanism of protection against the herbicide.