Cambios en el perfil de gangliósidos en distintas áreas cerebrales de ratas expuestas a una fracción parcialmente purificada de giroxina del veneno de Crotalus durissus terrificus

RUIZ DE TORRENT RAQUEL; BONGIOVANNI BETTINA; ACOSTA DE PEREZ OFELIA; DUFFARD RICARDO

Corrientes

Congreso; XXXIII Reunión Anual de la Asociación Argentina de Farmacología Experimental; 2001

Sociedad Argentina de Farmacología Experimental (SAFE)

A thrombin-like enzyme, purified from the venom of Crotalus durissus terrificus by gel filtration and affinity chromatography, showed a single protein band in Sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS-PAGE) with a molecular weight of about 33kDa. Clear cellular morphological changes, deep ganglioside level modifications in some brain areas and behavioral alterations in pup rats injected with this protein were detected. Ganglioside composition, one of the chemical markers of brain maturation, was altered specially in the hypothalamus, hippocampus and prefrontal cortex. The most reliable behavioral effects were a delayed, maturation of the righting reflex, posture and motor response after treatment. These effects were consistent with the histological changes revealed in the cerebellum and prefrontal cortex of treated neonate rats, areas related to motor activities.