Differential expression analysis of cold tolerance adaptation in D. buzzatii by RNA-seq de novo approach

NICOLÁS MOREYRA; JULIAN MENSCH; JUAN HURTADO; ESTEBAN HASSON

Conferencia; VI Argentinian Conference on Bioinformatics and Computational Biology; 2015

Many traits and biological processes can be affected by climatic changes and genetic variation has a major role in the ecological adaptation to thermal changes during development steps. Adaptation to these kinds of environments appears to involve a set of traits related to reproduction, stress resistance and metabolic processes. We have performed a RNA-seq analysis in Drosophila buzzatii to investigate profiles of gene expression changes during female reproductive diapause. We found 87 transcript differentially expressed into two clusters. All transcripts were blasted against genome assembly, annotated genes and non-redundant databases (Flybase and NCBI). The most relevant transcripts exhibited high identity to chorion protein coding genes of D. melanogaster, Vitelline membrane cysteine-rich domains, Heat Shock Protein (HSP) coding genes and critical respiratory chain genes of the mitochondrial genome (D. mojavensis).