Cultivable bacterial microbiota of roots, stalks and rhizosphere of Saccharum officinarum: a new reservoir of plant growth-promoting bacteria (PGPB)?

CINÀ P. ; ALIBRANDI P.; SIINO D.; LO MONACO N.; MICHAVILA M.G.; FILIPPONE M.P.; CASTAGNARO A.P. ; RACEDO, J.. ; GALLO G.; CIACCIO M. ; PUGLIA A.M.

Palermo

Encuentro; IBIM-CNR V° Meeting "Biotecnologie: ricerca di base, interdisciplinare e traslazionale in ambito biomedico".; 2018

IBIM-CNR

Cultivable bacterial microbiota of roots, stalks and rhizosphere of Saccharum officinarum: a new reservoir of plant growth-promoting bacteria (PGPB)?P. Cinà1, P. Alibrandi#2, D. Siino1, N. Lo Monaco1, G. Michavila3, M.P. Filippone3, A.P. Castagnaro3, J. Racedo3, G. Gallo1, M. Ciaccio4, A.M. Puglia11. Laboratory of Molecular Microbiology and Biotechnology, STEBICEF Dept, University of Palermo, Viale delle Scienze ed.16, 90128 Palermo, Italy2.torino3. Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Av. William Cross 3150/ CC: Nº9 CD (4101) Las Talitas, Tucumán.4. Istituto di Biomedicina e Immunologia Molecolare "A. Monroy" Consiglio Nazionale delle Ricerche CNR, Via La Malfa 153, 90146 Palermo, Italy#Co-first authorMicroorganisms are widespread inhabitants of rhizosphere and plant tissues and have been shown to assist plant growth and health. However, studies evaluating the diversity, structure and impact of the microbiota in economic important crops are still rare.In this work, we aimed to isolate, identify and characterize the microbiota, of roots, stalks, and rhizosphere of Saccharum officinarum a sugarcane widely cultivated in Argentine on industrial scale for sugar and biofuel production.A total of thirty-two strains were isolated from roots, stalks and rhizosphere of Saccharum officinarum which were taxonomically characterized on the base of their 16S rRNA. Furthermore, plant growth-promoting (PGP) properties of all the strains identified were assayed. The isolated strains differentially produced indole acetic acid (IAA) and siderophores. Moreover, they exhibited a variable capacity for phosphate solubilizations, nitrogen-fixing, antibiotic productions and resistance to the water and salt stresses.The results indicated that microbial endophytes isolated from S.officinarum may possess a vital role to improve plant growth and could be used as inoculants to establish a sustainable crop production system