Oxidativa burst in defense response in Fragaria x ananassa -Colletotrichum spp. interaction

TONELLO U.; RACEDO J.; SALAZAR S.; CASTAGNARO A.P.; DÍAZ RICCI J. C.

BIOCELL

Inca Editorial

Lugar: Buenos Aires; Año: 2006 vol. 30 p. 191 - 191

0327-9545

A rapid and transient generation of AOS (Active Oxygen Species) in F. x ananassa leaves challenges with an avirulent isolate of Colletotrichum sp. that produces an incompatible interaction, was observed. However, the latter phenomenon did not take place with fungal pathogens that produce compatible interactions. The aim of the present work was to demostrate the importance of this ?oxidative burst? in the establishment of defense response. In order to confirm this hypothesis, we have used the system glucose (2,5 mM)/glucose oxidase (2,5 U/mL) (G/GOD) to provide a sustained accumulation of H2O2 on leaves to mimic the pathogen- induced oxidative burst. Ours results showed that when the G/GOD system in applied simultaneously to the infection with the virulent pathogen that produces a compatible interaction, anthracnose symptoms were not observed in plants. However, when strawbery leaves were pretreated with the G/GOD system 1 h before the infection, the generation of H2O2 was enough to abolish the disease symptoms in strawberry.