GARCIA Daniela Celeste
congresos y reuniones científicas
BMP5 INDUCES IN VITRO MIGRATION OF BOVINE OVIDUCTAL EPITHELIAL CELLS
BRAVO-MIANA RC; GARCIA DANIELA; GARCÍA EV; ROLDAN-OLARTE EM; VALZ GIANINET JN; VALDECANTOS PA
Congreso; 52th Annual Meeting Argentine Society for Biochemistry and Molecular Biology, LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Bone morphogenetic proteins (BMPs) have an essential role in organogenesis and tissue repair. We have previously reported the expression of a number of BMPs and their receptors in the bovine oviduct epithelial cells (BOEC). The knowledge about their functions in the oviduct is scarce. In the present work, by using the in vitro wound healing assay, the potential role of BMP5, as an autocrine factor, was evaluated in primary BOEC cultures. In vitro cultured BOEC monolayers from isthmus were cultured for 24 h in serum-free medium in the presence of human rBMP5 (100 ng/ml) after a scratch made in the monolayers; the scratched area was measured at 0, 3, 6, 12 and 24 h (n=3) and compared with non-treated control groups. BMP5 increased significantly the cells ability to close the scratched area at 24 h. No significant differences between treated and control groups were observed until 12 h. RT-qPCR assays were performed to evaluate the ID2 and SMAD6 mRNA levels after 24 h of incubation. Although no significant differences were detected for these direct targets of BMP signaling, results suggest that BMP5 and other BMP should be autocrine factors regulating the repair and renovation of the bovine oviductal epithelium in physiological and pathological states.