GARCIA Daniela Celeste
congresos y reuniones científicas
DETECTION OF MRNA SEQUENCES OF PLASMINOGEN ACTIVATION SYSTEM COMPONENTS IN VICUÑA OVIDUCT
ROLDÁN-OLARTE, M; GARCÍA, D; APICHELA, SA ; MICELI, DC
Tafi del Valle, Tucumán
Jornada; XXVII Jornadas Científicas de la Asociación de Biología de Tucumán; 2010
Asociación de Biología de Tucumán
The mammalian oviduct is involved in the first stages of reproduction process. In previous studies we analyzed the activity of components of the Plasminogen Activation System in porcine oviduct, showing that plasminogen activators (PA) are expressed in it. One of them, the urokinase-type PA (u-PA), plays a key role in pericellular proteolysis, by binding to its receptor (u-PAR). In this work we analyzed if components of this proteolytic system are present in the vicuña oviduct. Total RNA was obtained from a primary oviductal epithelial cells culture. The expression of u-PA and u-PAR was performed by RT-PCR, using primers designed from sequences corresponding to other mammalian species. Amplification fragments were cloned, sequenced and analyzed with BLAST programs, confirming that they are high percentages of identity with sequences of u-PA and u-PAR in pig and cattle. Due that does not exist many vicuña codifying sequences in the databases, the informed sequences are an important contribution to the knowledge of the vicuña genome. The detection of u-PA and u-PAR mRNA fragments demonstrate that these genes are co-expressed in the vicuña oviductal cells. It suggests the participation of these components in the activation of molecules present in the oviductal lumen, involved in the events that take place in the oviduct.