GARCIA Daniela Celeste
congresos y reuniones científicas
DETECTION OF UROKINASE TYPE PLASMINOGEN ACTIVATOR RECEPTOR (U-PAR) IN OOCYTE AND PORCINE OVIDUCT
ROLDÁN-OLARTE, MARIELA; JIMÉNEZ-DÍAZ, MARÍA; GARCÍA, DANIELA; MICELI, DORA C.
Tafi del Valle, Tucumán
Jornada; XXIII JORNADAS CIENTÍFICAS DE LA ASOCIACIÓN DE BIOLOGÍA DE TUCUMÁN; 2006
Asociación de Biología de Tucumán
Plasminogen activators (PAs) are highly specific proteolytic enzymes that convert plasminogen (PIg) to plasmin (PI). Two types of PAs are know: tissue type (t-PA) and urokinase type (u-PA). The u-PA receptor (u-PAR) is present in the plasma membrane of several cell types, the u-PA/ u-PAR complex permits the focalized PI generation in a few areas of cellular surfaces and can active intracellular signal transduction system. We demostrated that porcine oviduct synthesizes PAs; in this work we analized the u-PAR presence in oocytes and porcine oviducts. Oocytes, obtained by aspiration of ovaries from recently slaughter housed sows, were selected, fixed and then analyzed by indirect immuno-fluorescence. An intense fluorescence over oocyte surface, but not in the zona pellucida was observed. Considering that PIg was previously detected in porcine oocytes, these results suggest that u-PA actives PIg near the oocyte surface. By indirect immunohisto-chemistry we also analyzed porcine oviducts, detecting u-PAR in epithelial and muscular cells but not in stroma cells from ampulla and isthmus. The presence of u-PAR in epithelial cells indicates that this molecule could bind the u-PA present in the oviductal lumen; the generation of PI on the epithelial surface and the activation of intracellular signals would be important to the fertilization or early embryo development.