Real-Time PCR detection of Paenibacillus larvae DNA from spores of scale samples

FERNANDEZ, N; QUINTANA, S.; ALVAREZ, B.; MEDICI, S.; GENDE, L; EGUARAS, M.

Mar del Plata

Congreso; X Congreso de Microbiologia General; 2014

SAMIGE

The gram-positive bacterium Paenibacillus larvae is a major pathogen of Apis mellifera, causing the disease known as American foulbrood. This condition affects the larval stage and causes as a final step dessication of the larvae leaving only a scale, which contains millions of bacterial spores. Spores of the microorganism initiate the infectious stage and are the major vectors for the spread of the disease. The delay in diagnosis causes the collapse of infected hives, therefore the development of a fast and relizable method of detection will be of a great help to prevent the spread of the disease. The objectve of this work was to develop a real-time PCR methodology for the detection of P. larvaefrom spores from scales samples.. Results: it was posible to apply a real-time PCR method for the detection of P. larvae