INVESTIGADORES
CAMARA Maria De Los Milagros
artículos
Título:
Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
Autor/es:
CAMARA MARIA DE LOS MILAGROSMARÍA DE LOS MILAGROS CAMARA, LEÓN A. BOUVIER, MARIANA R. MIRANDA,CHANTAL REIGADA Y CLAUDIO A. PEREIRA.
Revista:
FOLIA PARASITOLOGICA
Editorial:
FOLIA PARASITOLOGICA
Referencias:
Año: 2013
ISSN:
0015-5683
Resumen:
Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmid Trypanosoma cruzi (Chagas 1909), causative agent of Chagas disease, especially for those parasite strains maintained as epimastigotes for many years. In the present work, we propose a method for obtaining metacyclic trypomastigotes, which were capable of infecting mammalian cells, by simply lowering pH media. The collected amastigotes and trypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro. Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using this method, we were able to infect cells with transgenic parasites obtaining trypomastigotes and amastigotes using a neomycin resistant cell line.
