Abstract: Biofilm Formation of Naphthalene Degrading Strains Enhanced by Annonaceous Acetogenins and Some of their Acetylated Derivates

RODRIGUEZ CAZORLA LR; PARELLADA EA; CARTAGENA E; BARDÓN A; NESKE A.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2012

0327-9545

16.BIOFILM FORMATION OF NAPHTHALENE DEGRADINGSTRAINS ENHANCED BY ANNONACEOUS ACETOGENINSAND SOME OF THEIR ACETYLATED DERIVATESRodriguez Cazorla LR, Parellada EA, Cartagena E, Bardón A,Neske A.Instituto de Química Orgánica, Facultad de Bioquímica, Químicay Farmacia. UNT. Ayacucho 471. Tucumán. E-mail: aneske@fbqf.unt.edu.arAnnonaceous acetogenins (ACG) are secondary metabolites isolatedfrom the Annonaceae family. Some ACG positively interfere withquorum sensing mechanisms, increasing biofi lm formation of PAHsdegrading strains. We intend to investigate the infl uence of ACG andsome of their acetylated analogs on Pseudomonas plecoglossicidaJ26, PN5 and CN4 biofi lm formation by comparison with controlsof each strain grown in the absence of ACG. The biofi lm formationassay is based on the ability of bacteria to grow attached topolystyrene microplates. To measure biofi lm formation LB mediumwas supplemented with 5 μl of a 100 μg.ml-1 ACG ethanolic solution(2.5 μg.ml-1 fi nal concentration) and inoculated with an overnight LBculture of the strain. Biofi lm formation was quantifi ed by the additionof crystal violet and OD560nm measurements. Acetylated itrabinand rolliniastatin-2 showed signifi cant differences on PN5 biofi lmformation in comparison with their natural analogs (162/145% and154/128%, respectively; ethanol control, 100%). Therefore, theseacetylated ACG will be selected for future naphthalene degradationassays with the PN5 strain. These resultswould be promising forbiofi lm-mediated bioremediation.