Carbon and Nitrogen Sources Influence Tricalcium Phosphate Solubilization and Extracellular Phosphatase Activity by Talaromyces flavus

STEFANONI RUBIO, PABLO; GODOY, MS; DELLA MÓNICA, IVANA FLORENCIA; PETTINARI, MARÍA JULIA; GODEAS, ALICIA MARGARITA; SCERVINO, JOSÉ MARTÍN

CURRENT MICROBIOLOGY

SPRINGER

Lugar: Berlin; Año: 2016 vol. 72 p. 41 - 47

0343-8651

The aim of this work was to study phosphate(P) solubilization (and the processes involved in this event)by Talaromyces flavus (BAFC 3125) as a function of carbon and/or nitrogen sources. P solubilization was evaluatedin NBRIP media supplemented with different carbon(glucose, sorbitol, sucrose, and fructose) and nitrogen(L-asparagine, urea, ammonium sulfate (AS), and ammonium nitrate (AN) combinations. The highest P solubilization was related to the highest organic acid production(especially gluconic acid) and pH drop for those treatmentswhere glucose was present. Also P solubilization washigher when an inorganic nitrogen source was supplemented to the media when compared to an organic one.Although not being present an organic P source, phosphatase activity was observed. This shows that P mineralization and P solubilization can occur simultaneously, andthat P mineralization is not induced by the enzymesubstrate. The combination that showed highest P solubilization was for AN-glucose. The highest acid phosphataseactivity was for AS-fructose, while for alkaline phosphatase were for AS-fructose and AN-fructose. Acidphosphatase activity was higher than alkaline. P solubilization and phosphatase activity (acid and alkaline) wereinfluenced by the different carbon?nitrogen combinations.A better understanding of phosphate-solubilizing fungicould bring a better use of soil P.