NEONATAL INCREASED ASTHMA RISK CAUSED BY MATERNAL STRESS PERSISTS THROUGH ADULTHOOD AND PROPAGATES TO THE SECOND GENERATION

ALDIRICO MARÍA DE LOS ANGELES; FLORENCIA MAGALÍ GIORGIO; ARIADNA SOTO; MATÍAS DAMIÁN PERRONE SIBILIA; ARCÓN NADIA; MARIANO PICCHIO; SANCHEZ VANESA; RATTAY GUIDO; MORETTA R; MARTIN VALENTINA; ALEJANDRA GOLDMAN; FENOY IGNACIO

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología 2018; 2018

RATIONALE: We have shown that maternal stress duringpregnancy results in an increase of litter susceptibility to develop allergiclung inflammation. In this work we aim to study how stable is this phenomenon. METHODS: To see if the increased asthma risk continuesthrough adulthood, pregnant BALB/c mice(day 15) were subjected to restraintstress exposures. Pups were treated with two i.p.injection of ovalbumin (OVA)/alum(day 4 and 47), challenged with antigenaerosol(days 50-52) and euthanized(day 54) (SF). Negative controls includedpups of non-stressed dams subjected to the same protocol (CF) or i.p.sensitized and aerosol challenged with PBS(PBS). To evaluate the intergenerationalsusceptibility, 6-weeks female pups (F1) from stressed and non-stresseddams(F0) were mated. Their pups (F2) were treated with an i.p. injection ofOVA/alum(day 4) before antigen aerosol challenge(days 12-14) and euthanized(day16) (S). Negative controls included F2 from non-stressed F0 subjected to thesame protocol (C) or i.p. sensitized and aerosol challenged with PBS (PBS). RESULTS: In adult F1, bronchoalveolar lavage (BAL) ofstressed-dams female progeny had increased eosinophils percentage compared tonon-stressed dams progeny (SF=15.53±2.942/CF=1.825±0.648)(p