B lineal epitopes prediction of Blomia tropicalis major allergens

CONSTANZA GUERRIERI MAGRINI; FEDERICO PÁEZ CÓRDOBA; GUIDO SCARPATI SOTO; ALEJANDRA GOLDMAN; IGNACIO FENOY

Mar del Plata

Congreso; Reunion Anual Biociencias 2022- reunion SAI FAIC; 2022

RATIOLE: House dust mite (HDM) allergy is the leading cause of IgE-mediated hypersensitivity. Blomia tropicalis has been reported to be a clinically important HDM allergen. As climate change has widened B.tropicalis geographic distribution its study is important in Argentina. Current diagnostic and therapy relay in whole mite extracts. There is an impossibility to obtain homogeneous allergen extract preparations from natural sources. To develop better diagnoses and immunotherapies against allergies there is a need to better understand allergen´s immunogenicity. We aim to predict linear B epitopes in B. tropicalis allergens. METHODS: B. tropicalis allergens were retrieved from allergen.org. Protein sequences were analyzed for post-translational modifications. Reported epitopes of B. tropicalis were retrieved from IEDB.org. B cell linear epitopes (lep) of each major allergen of B. tropicalis were predicted using 8 software according to the following pipeline: i) ABCpred with a 16-mer and a 0.75 threshold; ii), found leps must also be identified by Bepipred Linear Epitope Prediction 2.0 or by Bepipred Linear Epitope Prediction. For this predicted lep must have more of 4 adjacent residues in at least one of these algorithms using default thresholds; and iii) selected epitopes must also be predicted by at least 3 of the following software: Chou & Fasman, Emini, Karplus & Schulz, Kolaskar & Tongaonkar and Parker (IEDB.org). RESULTS: For B. tropicalis 14 major allergens were found (Blo t 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 19, 21). Only Blo t 5, 21 13 have reported lep in IEDB. After applying the described pipeline we found the following lep: Blo t 21 (1), Blo t 1 (15), Blo t 11 (29), Blo t 3 (12), Blo t 12 (7), Blo t 7 (5), Blo t 5 (1), Blo t 8 (6), Blo t 4 (19), Blo t 10 (9), Blo t 13 (7), Blo t 19 (2), Blot 6 (14) and Blo t 2 (7). These epitopes could be useful for allergy immunotherapy and diagnosis development.