Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin B1 adsorbents

MAGNOLI, ALEJANDRA.; RODRIGUEZ, MARINA; POLONI, VALERIA; ROJO, MARÍA; COMBINA, MARIANA; CHIACCHIERA, STELLA.; DALCERO, ANA.; CAVAGLIERI, LILIA.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2016

1364-5072

Aims: isolate and characterize native yeast strains from broilers environment as feedstuff,faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of 9 yeast strains were isolated: 3 from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), 2 from gut identified as Candida tropicalis and 4 from faeces identified as C. lusitaniae (3) and Cyberlindnera fabianii (1).AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. For adsorption studies, the most appropriate concentrations for cells and toxin were 107cell ml-1 30 and 100 ng ml-1 31 of AFB1, respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherms studies in all yeast assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the complex AFB1 yeast demonstrated the irreversibility of the binding process.Conclusion: Yeast strains tested in the present work constitute potential AFB1 adsorbents because they possess the advantage to be native from the avian environment. Significance and Impact of the Study: This manuscript makes a contribution on using of native yeasts from broilers environment for controlling chronic aflatoxicosis in avian production.