Genome analysis of the thermoacidophilic archaeon Acidianus copahuensis focusing on the metabolisms associated to biomining activities

URBIETA, MARÍA SOFÍA; RASCOVAN, NICOLÁS; VÁZQUEZ, MARTÍN P.; DONATI, EDGARDO

BMC GENOMICS

BIOMED CENTRAL LTD

Año: 2017 vol. 18

1471-2164

Background: Several archaeal species from the order Sulfolobales are interesting from the biotechnological pointof view due to their biomining capacities. Within this group, the genus Acidianu s contains four biomining species(from ten known Acidianus species), but none of these have their genome sequenced. To get insights into thegenetic potential and metabolic pathways involved in the biomining activity of this group, we sequenced thegenome of Acidianus copahuensis ALE1 strain, a novel thermoacidophilic crenarchaeon (optimum growth: 75 °C,pH 3) isolated from the volcanic geothermal area of Copahue at Neuquén province in Argentina. Previousexperimental characterization of A. copahuensis revealed a high biomining potential, exhibited as high oxidationactivity of sulfur and sulfur compounds, ferrous iron and sulfide minerals (e.g.: pyrite). This strain is also autotrophicand tolerant to heavy metals, thus, it can grow under adverse conditions for most forms of life with a low nutrientdemand, conditions that are commonly found in mining environments.Results: In this work we analyzed the genome of Acidianus copahuensis and describe the genetic pathwaysinvolved in biomining processes. We identified the enzymes that are most likely involved in growth on sulfur andferrous iron oxidation as well as those involved in autotrophic carbon fixation. We also found that A. copahuensisgenome gathers different features that are only present in particular lineages or species from the order Sulfolobales,some of which are involved in biomining. We found that although most of its genes (81%) were found in at leastone other Sulfolobales species, it is not specifically closer to any particular species (60?70% of proteins shared witheach of them). Although almost one fifth of A. copahuensis proteins are not found in any other Sulfolobales species,most of them corresponded to hypothetical proteins from uncharacterized metabolisms.Conclusion: In this work we identified the genes responsible for the biomining metabolisms that we havepreviously observed experimentally. We provide a landscape of the metabolic potentials of th is strain in the contextof Sulfolobales and propose various pathways and cellular processes not yet fully understood that can use A.copahuensis as an experimental model to further understand the fascinating biology of thermoacidophilicbiomining archaea.