Polyphenols from cafayate grape pomace display high inhibitory activity on human acetylcholinesterase

SALAZAR, PB; MINAHK, C; RODRIGUEZ VAQUERO, MJ

Bellevue, Washington

Congreso; 68th ASEV National Conference; 2017

American Society for Enology and Viticulture

p { margin-bottom: 0.25cm; direction: ltr; line-height: 120%; text-align: left; }Polyphenolsare ubiquitous plant metabolites that have been the subject ofincreasing interest due to their benefits on human health. Theinhibition of acetylcholinesterase activity represents a verypromising activity because it may have great impact in severalpathologies such as myasthenia gravis, glaucoma and particularlyAlzheimer´s disease. Theaim of this study was to analyze the effect of phenolics-richextracts from winerywastesonhuman acetylcholinesterase activity. For this purpose, grape pomaceof Torrontes and Malbec grape varieties was obtained from a Cafayatewinery (Valles Calchaquíes, Argentina). From each sample, amethanolic and an ethyl-acetate extracts were obatined using soxhlet.The total phenolic content and a preliminary phenolic profile in eachwaste sample were evaluated using Folin-Ciocalteureagentand TLC assay. The acetylcholinesterase (AChE) activity of differentextracts and pure phenolic compounds was determined by Ellman´scolorimetric method and also using indoxylacetateasthe chromogenic substrate. Human red blood cell acetylcholinesterasein its membrane-bound form was used as the target enzyme, becausethis isoform proved to be a good model of the more important isoformsassociated for instance to Alzheimer´s and myasthenia gravis. Theresults presented here allow us to conclude that both grape pomacevarieties extracts display a noticeable inhibition towards AChE,being the red grape pomace more effective to produce the enzymeactivity. These findings suggest that waste material from thewinemaking industry could be a promising source of anticholinesteraseagents. These by-products will certainly have high added value, whichin turn will give a positive impact in the regional economy.