Parallel screening and optimization of protein constructs for structural studies

RASIA RM; NOIRCLERC-SAVOYE M; BOLOGNA NG; GALLET B; PLEVIN MJ; BLANCHARD L; PALATNIK JF; BRUTSCHER B; VERNET T; BOISBOUVIER J

PROTEIN SCIENCE

JOHN WILEY & SONS INC

Año: 2009 vol. 18 p. 434 - 439

0961-8368

A major challenge in structural biology remains the identification of protein constructs amenable to structural characterization. Here, we present a simple method for parallel expression, labeling, and purification of protein constructs (up to 80 kDa) combined with rapid evaluation by NMR spectroscopy. Our approach, which is equally applicable for manual or automated implementation, offers an efficient way to identify and optimize protein constructs for NMR or X-ray crystallographic investigations.