Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses

OLIVERA, RAMIRO; MORO, LUCIA; JORDAN, ROBERTO; PALLAROLS, NATALIA; GUGLIELMINETTI, ALEJANDRO; LUZZANI, CARLOS; MIRIUKA, SANTIAGO; VICHERA, GABRIEL

Stem Cells and Cloning: Advances and Applications

Dove Press

Lugar: Auckland; Año: 2018 vol. Volume 11 p. 13 - 22

IntroductionCell plasticity is crucial in cloning to allow an efficient nuclear reprogramming and healthy offspring. Hence, cells with high plasticity, such as multipotent mesenchymal stem cells (MSCs), may be a promising alternative for horse cloning. In this study, we evaluated the use of bone marrow-MSCs (BM-MSCs) as nuclear donors in horse cloning, and we compared the in vitro and in vivo embryo development with respect to fibroblasts.Materials and methodsZona-free nuclear transfer was performed using BM-MSCs (MSC group, n=3432) or adult fibroblasts (AF group, n=4527). Embryos produced by artificial insemination (AI) recovered by uterine flushing and transferred to recipient mares were used as controls (AI group).ResultsBlastocyst development was higher in the MSC group than in the AF group (18.1% vs 10.9%, respectively; p