Tissue transglutaminase expressed in trophoblast cells as a possible target of auto antibodies contributing to pregnancy complications of celiac patients

C. SÓÑORA; G. CALO; L. FRACCAROLI; V. HAUK; A. HERNÁNDEZ; C. PÉREZ LEIRÓS; R. RAMHORST

Congreso; V SLIMP - IV LASRI Meeting 2013; 2013

Background: Celiac disease is associated with pregnancy complications. Antibodies against tissue transglutaminase (tTG) could mediate pathogenic mechanisms since tTG has multiple functions at maternal-fetal interface. We analized if autoantibodies affect trophoblast migration and apoptotic cell phagocytosis by macrophages. Methods: An injury was generated in conuent trophoblast monolayer (Swan-71) and wound healing assessed by microscopy at 4-8-24h in presence of MoAb-TG2 (10ug/ml), celiac or normal women sera (1:50). Phagocytosis using differentiated THP-1 and apoptotic Swan-71cells (1:1) were performed with MoAb-TG2 or women sera. Phagocytosis percentage was determined by ow cytometry CD14þCFSEþ cell counting. Protocols were approved by School of Medicine-UDELAR, Ethics Committee. Results: AcMo-TG2 and celiac women sera delayed injury healing compared to normal sera. AcMo-TG2 and some celiac sera but not normal sera inhibited phagocytosis of apoptotic trophoblast cells. Conclusions: These observations suggest that anti-tTG antibodies could inhibit trophoblast cell migration and apoptotic trophoblast cell clearance by macrophages at the maternal-fetal interface.