A potential tolerogenic immune mechanism by trophoblast cells through the activation of chemokine-induced T cell death and regulatory T cell modulation

L. FRACCAROLI; J. ALFIERI; L. LAROCCA; M. CALAFAT; G. MOR; C. PÉREZ LEIRÓS; R. RAMHORST

HUMAN REPRODUCTION

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2009 vol. 24 p. 166 - 175

0268-1161

BACKGROUND: Successful implantation is followed by a local pro-inflammatory and Th1 response, subsequently controlled by Th2. Regulated upon activation, normal T cell expressed and secreted (RANTES) promotes a Th1 response and is implicated as a physiologic tolerogenic factor; therefore, we studied its potential role in the trophoblast–maternal leukocyte dialog. METHODS: We performed co-cultures of immortalized trophoblast cell line (Swan 71) and peripheral blood mononuclear cells (PBMCs) from fertile women (n=5) or with recurrent spontaneous abortions (n=5, RSA). After 24 and 48 h, supernatant and cells were analyzed by enzyme-linked immunosorbent assay, fluorescence-activated cell sorting, Western blot and apoptosis assay. To investigate the physiological effects at peripheral level, we co-cultured maternal and paternal PBMCs with conditioned media from Swan cells and progesterone. RESULTS: Following interaction of maternal PBMCs and trophoblast cells, RANTES production increased (P