B cells regulate the inflammatory CD4+ T cell response in Trypanosoma cruzi infection.

GOROSITO SERRÁN M; TOSELLO BOARI J; RAMELLO MC; BECCARIA CG; FIOCCA VERNENGO F; BERMEJO DA; AMEZCUA VESELY MC; MONTES CL; ACOSTA RODRIGUEZ EV; GRUPPI A

Congreso; LVII Reunión Anual de la Sociedad Argentina de Inmunología; 2014

B cell-deficient mice (mMT) infected with T. cruzi are able to control parasitemia similar to C57BL/6 wild type (WT) mice but present increased parasitemia by 10 days post-infection (dpi) and accelerated mortality. mMT and WT mice have similar tissue parasite load as determined by RT-PCR, indicating that mortality is not caused by uncontrolled parasitism. To assess possible causes of the increased susceptibility of mMT mice, we analyzed cytokine production and CD4+T cell responses in mMT and WT mice infected i.p. with 10000 trypomastigotes Y-br strain. Groups of 4-7 mice were sacrificed by 4, 9, 15, 20 dpi and sera and splenocytes were obtained. By 9 dpi, amounts of serum IFNg were increased in both infected mice strains, but were higher in mMT mice (p