B CELL-DEFICIENT MICE INFECTED WITH TRYPANOSOMA CRUZI DEVELOP A PRO-INFLAMMATORY T CELL RESPONSE POTENTIALLY HARMFUL FOR THE HOST.

GOROSITO SERRÁN M; TOSELLO BOARI J; BERMEJO DA; RAMELLO MC; FIOCCA VERNENGO F; AMEZCUA VESELY MC; BECCARIA CG; MONTES CL; ACOSTA RODRIGUEZ EV; GRUPPI A

Congreso; First Argentinean Spring Course in Advance Immunology and LXI Reunión Anual de la Sociedad Argentina de Inmunología.; 2013

B cell-deficient mice (C129.BL6mMT) infected with T. cruzi, have more parasitemia than C57BL/6 wild type (WT) mice and accelerated mortality. To assess possible causes of this increased susceptibility, we analyzed the production of cytokines and the CD4+T-cell response in infected mMT and WT mice. Mice were infected by i.p. injection of 10000 trypomastigotes Y-br strain. Groups of 4-7 mice were sacrificed on days 4, 9, 15, 20 post-infection (pi); splenocytes were obtained and CD4+ T cells were studied by flow-cytometry. In addition, serum samples were obtained to quantify IFNg and TNF by ELISA. Both infected mice strains presented by day 9 (pi) increased amounts of serum IFNg, being higher in mMT mice (p