Early Treg cell depletion during Trypanosoma cruzi infection promotes Tconv and CD8+ T cell immunity in the acute phase.

ARAUJO FURLÁN CL; BOCCARDO S; RODRIGUEZ C; MONTES CL; GRUPPI A; ACOSTA RODRIGUEZ EV

Congreso; Reunión Conjunta SAIC.SAI.AAFE.NANOMED.Ar.; 2021

We reported that after Trypanosoma cruzi (Tc) infection, Tregs undergoa marked and sustained reduction in frequency. This naturalcontraction of the Treg response was critical to allow the emergenceof protective anti-parasite CD8+T cell immunity in the acute phase.In line with this, we previously demonstrated that Treg depletion atday (d) 5 post-infection (pi) but not at d11pi impacted on the magnitudeof anti-parasite CD8+T cell response and the ability to controlparasite replication in the acute phase. Thus, we hypothesized thatTregs may exert a role during early events of T cell priming. In orderto assess this, DEREG mice were infected with Tc and injected withdiphtheria toxin (DT) or PBS at d5 and 6pi. However, DT treatmentonly induced modest effects on APCs shortly after the injection.Specially, CD86 expression was upregulated on splenic DCs, macrophagesand NKT cells of DT-injected mice in contrast to controls(p