Phenotypic and functional identification of human IL-17 producing memory T cells?

ACOSTA RODRIGUEZ EV; RIVINO L; GEGINAT J; JARROSSAY D; GATTORNO M; LANZAVECCHIA A; SALLUSTO F

NATURE IMMUNOLOGY (PRINT)

Año: 2007 p. 639 - 646

1529-2908

Interleukin 17 (IL-17)?producing T helper cells (TH-17 cells) have been characterized in mice as a distinct subset of effector cells, but their identity and properties in humans remain elusive. We report here that expression of CCR6 and CCR4 together identified human memory CD4+ T cells selectively producing IL-17 and expressing mRNA encoding the human ortholog of mouse RORct, a transcription factor, whereas CCR6 and CXCR3 identified TH1 cells producing interferon-c and T helper cells producing both interferon-c and IL-17. Memory T cells specific for Candida albicans were present mainly in the CCR6+CCR4+H-17 cells) have been characterized in mice as a distinct subset of effector cells, but their identity and properties in humans remain elusive. We report here that expression of CCR6 and CCR4 together identified human memory CD4+ T cells selectively producing IL-17 and expressing mRNA encoding the human ortholog of mouse RORct, a transcription factor, whereas CCR6 and CXCR3 identified TH1 cells producing interferon-c and T helper cells producing both interferon-c and IL-17. Memory T cells specific for Candida albicans were present mainly in the CCR6+CCR4++ T cells selectively producing IL-17 and expressing mRNA encoding the human ortholog of mouse RORct, a transcription factor, whereas CCR6 and CXCR3 identified TH1 cells producing interferon-c and T helper cells producing both interferon-c and IL-17. Memory T cells specific for Candida albicans were present mainly in the CCR6+CCR4+ct, a transcription factor, whereas CCR6 and CXCR3 identified TH1 cells producing interferon-c and T helper cells producing both interferon-c and IL-17. Memory T cells specific for Candida albicans were present mainly in the CCR6+CCR4+c and IL-17. Memory T cells specific for Candida albicans were present mainly in the CCR6+CCR4+ TH-17 subset, whereas memory T cells specific for Mycobacterium tuberculosis were present in CCR6+CXCR3+ T helper type 1 subset. The elicitation of IL-17 responses correlated with the capacity of C. albicans hyphae to stimulate antigen-presenting cells for the priming of TH-17 responses in vitro and for the production of IL-23 but not IL-12. Our results demonstrate that human TH-17 cells have distinct migratory capacity and antigenic specificities and establish a link between microbial products, T helper cell differentiation and homing in response to fungal antigens.H-17 subset, whereas memory T cells specific for Mycobacterium tuberculosis were present in CCR6+CXCR3+ T helper type 1 subset. The elicitation of IL-17 responses correlated with the capacity of C. albicans hyphae to stimulate antigen-presenting cells for the priming of TH-17 responses in vitro and for the production of IL-23 but not IL-12. Our results demonstrate that human TH-17 cells have distinct migratory capacity and antigenic specificities and establish a link between microbial products, T helper cell differentiation and homing in response to fungal antigens.C. albicans hyphae to stimulate antigen-presenting cells for the priming of TH-17 responses in vitro and for the production of IL-23 but not IL-12. Our results demonstrate that human TH-17 cells have distinct migratory capacity and antigenic specificities and establish a link between microbial products, T helper cell differentiation and homing in response to fungal antigens.H-17 responses in vitro and for the production of IL-23 but not IL-12. Our results demonstrate that human TH-17 cells have distinct migratory capacity and antigenic specificities and establish a link between microbial products, T helper cell differentiation and homing in response to fungal antigens.H-17 cells have distinct migratory capacity and antigenic specificities and establish a link between microbial products, T helper cell differentiation and homing in response to fungal antigens.