Ubiquitin-like proteins in halobacteria

DEBORA NERCESSIAN; DE-CASTRO, R. E.; CONDE, R. D.

JOURNAL OF BASIC MICROBIOLOGY

WILEY-V C H VERLAG GMBH

Lugar: Berlin; Año: 2002 vol. 42 p. 277 - 283

0233-111X

Within our studies of protein degradation, the presence of ubiquitinylated proteins in haloalkaliphilicarchaea was investigated. We found that Natronococcus occultus proteins that react with antibodiesraised against ubiquitin appear in different growth phases, particularly in the initial and exponentialones. The expression of these proteins is increased when the cells are either treated with puromycin or starved for nutrients. Dot blot analysis of cell extracts with antibody against ubiquitin shows thepresence of either ubiquitinylated or ubiquitin-like proteins not only in Natronococcus occultus, butalso in various genera of halobacteria.